OPTIMIZATION OF THERMOSTABLE ALPHA-AMYLASE PRODUCTION FROM GEOBACILLUS SP. D413

Production of Thermostable α-Amylase and Cellulase from Cellulomonas sp

A bacterium, isolated from rabbit’s waste and identified as Cellulomonas sp., had cellulase and thermostable α-amylase activity when grown on wheat bran. Maximum activity of thermostable α-amylase was obtained by adding 3% soluble starch. However, soybean oil (1 ml l) could increase the production of α-amylase and cellulase in wheat bran. The α-amylase was characterized by making a demonstratio...

Highly Thermostable Xylanase Production from A Thermophilic Geobacillus sp. Strain WSUCF1 Utilizing Lignocellulosic Biomass

Efficient enzymatic hydrolysis of lignocellulose to fermentable sugars requires a complete repertoire of biomass deconstruction enzymes. Hemicellulases play an important role in hydrolyzing hemicellulose component of lignocellulose to xylooligosaccharides and xylose. Thermostable xylanases have been a focus of attention as industrially important enzymes due to their long shelf life at high temp...

Production of a thermostable extracellular amylase from thermophilic Bacillus species

Isolation and identification of thermophilic Bacillus sp was carried out from a soil sample. The cells were cultivated in a medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the medium shortened the lag period and improved the growth and amylase synthesis. The optimum temperature for amylase production was detecte...

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

Highly thermostable GH39 β-xylosidase from a Geobacillus sp. strain WSUCF1

BACKGROUND Complete enzymatic hydrolysis of xylan to xylose requires the action of endoxylanase and β-xylosidase. β-xylosidases play an important part in hydrolyzing xylo-oligosaccharides to xylose. Thermostable β-xylosidases have been a focus of attention as industrially important enzymes due to their long shelf life and role in the relief of end-product inhibition of xylanases caused by xylo-...